Regulation of neuronal progenitor delamination by dynein-driven post-Golgi apical transport
Brault, J.-B.; Bardin, S.; Lampic, M.; Carpentieri, J.; Coquand, L.; Penisson, M.; Lachuer, H.; Victoria, G. S.; Baloul, S.; Boncompain, G.; Miserey-Lenkei, S.; Fraisier, V.; Francis, F.; Perez, F.; Goud, B.; Baffet, A. D.
Show abstract
Radial glial (RG) cells are the neural stem cells of the developing neocortex. Apical RG (aRG) cells can delaminate to generate basal RG (bRG) cells, a cell type associated with human brain expansion. Here, we report that this delamination is regulated by the post-Golgi secretory pathway. Using in situ subcellular live imaging, we show that post-Golgi transport of RAB6+ vesicles occurs toward the minus ends of microtubules and depends on dynein. We demonstrate that the apical determinant Crumbs3 (CRB3) is also transported by dynein. Double knockout of RAB6A/A and RAB6B impairs apical localization of CRB3, and induces a retraction of aRG cell apical process, leading to delamination and ectopic division. These defects are phenocopied by knock-out of the dynein activator LIS1. Overall, our results identify a RAB6-dynein-LIS1 complex for Golgi to apical surface transport in aRG cells, and highlights the role of this pathway in the maintenance of neuroepithelial integrity.
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