The RNA m6A binding protein YTHDF2 promotes the B cell to plasma cell transition.
Turner, D.; Saveliev, A.; Salerno, F.; Matheson, L. S.; Screen, M.; Lawson, H.; Wotherspoon, D.; Kranc, K.; Turner, M.
Show abstract
To identify roles of RNA binding proteins (RBPs) in the differentiation of B cells to antibody-secreting plasma cells we performed a CRISPR/Cas9 knockout screen of 1213 mouse RBPs for their ability to affect proliferation and/or survival, and the emergence of differentiated CD138+ cells in vitro. We identified RBPs that promoted the appearance of CD138+ cells including CSDE1 and STRAP, as well as RBPs that inhibited CD138+ cell appearance such as EIF3 subunits EIF3K and EIF3L. Furthermore, we identified RBPs that share the property of recruiting the CCR4-NOT complex to their target transcripts have the potential to mediate opposing outcomes on B cell differentiation. One such RBP, the m6A binding protein YTHDF2 promotes the appearance of CD138+ cells in vitro. In chimeric mouse models YTHDF2-deficient B cells formed germinal centers in a cell-autonomous manner, however plasma cells failed to accumulate.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- An integrated proteome and transcriptome of B cell maturation defines poised activation states of transitional and mature B cells 97%
- B1 and B2 B cells are characterized by distinct CpG modification states at DNMT3A-maintained enhancers 97%
- Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow 97%
Similar papers in this journal
- A negative feedback loop mediated by the NR4A family of nuclear hormone receptors restrains expansion of B cells that receive signal one in the absence of signal two 97%
- The transcription factor Hhex cooperates with the corepressor Tle3 to promote memory B cell development 96%
- Stepwise chromatin and transcriptional acquisition of an intraepithelial lymphocyte program 95%
Similar papers in this journal
- Systematic identification of cancer cell vulnerabilities to natural killer cell-mediated immune surveillance 96%
- SLAM/SAP signaling regulates discrete γδ T cell developmental checkpoints and shapes the innate-like γδ TCR repertoire 96%
- Foxp3 depends on Ikaros for control of regulatory T cell gene expression and function 96%
Similar papers in this journal
- CD20 as a gatekeeper of the resting stage of human B cells 96%
- The histone methyltransferase DOT1L prevents antigen-independent differentiation and safeguards epigenetic identity of CD8+ T cells 96%
- Genomic Analysis of Progenitors in Viral Infection Implicates Glucocorticoids as Suppressors of Plasmacytoid Dendritic Cell Generation 96%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.