Single-cell bisulfite sequencing of spermatozoa from lean and obese humans reveals potential for the transmission of epimutations
Barres, R.; Andersen, E.; Reik, W.; Clark, S.; Ingerslev, L.; Lundell, L.
Show abstract
Epigenetic marks in gametes modulate developmental programming after fertilization. Spermatozoa from obese men exhibit distinct epigenetic signatures compared to lean men, however, whether epigenetic differences are concentrated in a sub-population of spermatozoa or spread across the ejaculate population is unknown. Here, by using whole-genome single-cell bisulfite sequencing on 87 motile spermatozoa from 8 individuals (4 lean and 4 obese), we found that spermatozoa within single ejaculates are highly heterogeneous and contain subsets of spermatozoa with marked imprinting defects. Comparing lean and obese subjects, we discovered methylation differences across two large CpG dense regions located near PPM1D and LINC01237. These findings confirm that sperm DNA methylation is altered in human obesity and indicate that single ejaculates contain subpopulations of spermatozoa carrying distinct DNA methylation patterns. Distinct epigenetic patterns of spermatozoa within an ejaculate may result in different intergenerational effects and therefore influence strategies aiming to prevent epigenetic-related disorders in the offspring.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Microhomology-Mediated Circular DNA Formation from Oligonucleosomal Fragments During Spermatogenesis 95%
- A spontaneous genetically-induced epiallele at a retrotransposon shapes host genome function 95%
- Dynamics of transcriptional programs and chromatin accessibility in mouse spermatogonial cells from early postnatal to adult life 95%
Similar papers in this journal
- Discovery of an unusual high number of de novo mutations in sperm of older men using duplex sequencing 94%
- Cell type- and chromosome-specific chromatin landscapes and DNA replication programs of Drosophila testis tumor stem-cell like cells 94%
- Gene essentiality in cancer cell lines is modified by the sex chromosomes 94%
Similar papers in this journal
- Epididymal acquired sperm microRNAs modify post-fertilization embryonic gene expression 95%
- Dissecting Mammalian Spermatogenesis Using Spatial Transcriptomics 94%
- Establishment of 3D chromatin structure after fertilization and the metabolic switch at the morula-to-blastocyst transition require CTCF 93%
Similar papers in this journal
- Widespread allele-specific topological domains in the human genome are not confined to imprinted gene clusters. 94%
- An atlas of lamina-associated chromatin across twelve human cell types revealsan intermediate chromatin subtype 93%
- Multi-omics analysis in primary T cells elucidates mechanisms behind disease associated genetic loci 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.