Single nucleus pituitary transcriptomic and epigenetic landscape reveals human stem cell heterogeneity with diverse regulatory mechanisms
Zhang, Z.; Zamojski, M.; Smith, G. R.; Willis, T. L.; Yianni, V.; Mendelev, N.; Pincase, H.; Seenarine, N.; Amper, M. A. S.; Vasoya, M.; Nair, V.; Turgeon, J. L.; Bernard, D. J.; Troyanskaya, O. G.; Andoniadou, C. L.; Sealfon, S. C.; Ruf-Zamojski, F. M.
Show abstract
Despite their importance in tissue homeostasis and renewal, human pituitary stem cells (PSCs) are incompletely characterized. We describe a human single nucleus (sn) RNAseq and ATACseq resource from pediatric, adult, and aged pituitaries (snpituitaryatlas.princeton.edu) and characterize cell type-specific gene expression and chromatin accessibility programs for all major pituitary cell lineages. We identify uncommitted PSCs, committing progenitor cells, and sex differences. Pseudotime trajectory analysis indicates that early life PSCs are distinct from the other age groups. Linear modeling of same-cell multiome data identifies regulatory domain accessibility sites and transcription factors (TFs) that are significantly associated with gene expression in PSCs compared to other cell types and within PSCs. Modeling the heterogeneous expression of two markers for committing cell lineages among PSCs shows significant correlation with regulatory domain accessibility for GATA3, but with TF expression for POMC. These findings characterize human stem cell lineages and reveal diverse mechanisms regulating key PSC genes.
Matching journals
The top 3 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Cis-regulatory architecture of human ESC-derived hypothalamic neuron differentiation aids in variant-to-gene mapping of relevant common complex traits 96%
- A human neural crest model reveals the developmental impact of neuroblastoma-associated chromosomal aberrations 96%
- Single-molecule long-read sequencing reveals a conserved selection mechanism determining intact long RNA and miRNA profiles in sperm 95%
Similar papers in this journal
Similar papers in this journal
- Defining the cellular origin of seminoma by transcriptional and epigenetic mapping to the normal human germline 96%
- Neural stem cell quiescence is actively maintained by the epigenome 95%
- Interrogation of cancer gene dependencies reveals novel paralog interactions of autosome and sexchromosome encoded genes 95%
Similar papers in this journal
- L1 retrotransposons drive human neuronal transcriptome complexity and functional diversification 96%
- Chronic exposure to glucocorticoids amplifies inhibitory neuron cell fate during human neurodevelopment in organoids 96%
- Single-cell genomics reveals region-specific developmental trajectories underlying neuronal diversity in the human hypothalamus 95%
Similar papers in this journal
- Electrophysiological measures from human iPSC-derived neurons are associated with schizophrenia clinical status and predict individual cognitive performance 94%
- Oct4 regulates embryonic pluripotency via metabolic mechanisms and Stat3 signalling. 94%
- PGC-1a drives small cell neuroendocrine cancer progression towards an ASCL1-expressing subtype with increased mitochondrial capacity 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.