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Efficient discovery of potently neutralizing SARS-CoV-2 antibodies using LIBRA-seq with ligand blocking

Shiakolas, A. R.; Johnson, N.; Kramer, K. J.; Suryadevara, N.; Wrapp, D.; Periasamy, S.; Pilewski, K. A.; Raju, N.; Nargi, R.; Sutton, R. E.; Walker, L.; Setliff, I.; Crowe, J. E.; Bukreyev, A.; Carnahan, R. H.; McLellan, J. S.; Georgiev, I. S.

2021-06-30 immunology
10.1101/2021.06.02.446813 bioRxiv
Show abstract

SARS-CoV-2 therapeutic antibody discovery efforts have met with notable success but have been associated with a generally inefficient process, requiring the production and characterization of exceptionally large numbers of candidates for the identification of a small set of leads. Here, we show that incorporating antibody-ligand blocking as part of LIBRA-seq, the high-throughput sequencing platform for antibody discovery, results in efficient identification of ultra-potent neutralizing antibodies against SARS-CoV-2. LIBRA-seq with ligand blocking is a general platform for functional antibody discovery targeting the disruption of antigen-ligand interactions.

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