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Ground tissue circuitry regulates organ complexity in cereal roots

Ortiz-Ramirez, C. H.; Dias Araujo, P. C.; Zhang, S.; Demesa-Arevalo, E.; Yan, Z.; Xu, X.; Rahni, R.; Gingeras, T. R.; Jackson, D.; Gallagher, K. L.; Birnbaum, K. D.

2021-04-29 genomics
10.1101/2021.04.28.441892 bioRxiv
Show abstract

Most plant roots have multiple cortex layers that make up the bulk of the organ and play key roles in physiology, such as flood tolerance and symbiosis. However, little is known about the formation of cortical layers outside of the highly reduced anatomy of the model Arabidopsis. Here we use single-cell RNAseq to rapidly generate a cell resolution map of the maize root, revealing an alternative configuration of the tissue formative SHORT-ROOT (SHR) signaling pathway adjacent to an expanded cortex. We show that maize SHR protein is hypermobile, moving at least eight cell layers into the cortex. Higher-order SHR mutants in both maize and Setaria have reduced numbers of cortical layers, showing that the SHR pathway controls expansion of cortical tissue in grasses that sets up anatomical complexity and a host of key traits. One sentence summarySingle-cell RNA-seq maps the maize root transcriptome uncovering a mechanism that regulates cortex layer number.

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