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Sesamol and its derivative investigated as antiandrogen - A potential prevention to prostate cancer in rats

Shah, A.; Shah, A. A.; Rekunge, D. S.; Pai, A.; Chaturbhuj, G. U.; K, N.; Lobo, R.

2021-04-23 cancer biology
10.1101/2021.04.22.440973 bioRxiv
Show abstract

Androgen signaling is essential for the development of prostate cancer (PCa) initiated from prostatic basal cells with collocation of androgen receptor gene mutations. Phytoestrogens, the naturally occurring compounds are AR antagonist. These compounds downregulate prostate-specific antigen (PSA) expression and cell proliferation. Thus, this gives a track to research these compounds as a possible treatment for PCa. In this work, STITCH and molecular docking predict the conformation of ligands inside the suitable target binding site. Therefore, a study was planned to know the interactions among SM and its derivatives with AR. It was further, evaluated for in vitro evaluation on LNCaP, PC-3, and DU-145 using MTT studies. The two lead compounds shortlisted from MTT studies were further analyzed for androgen-regulated genes by using RT-PCR, western blot studies and an animal model of prostate cancer. We found that SM and its derivative (3-MA) may prevent the development of PCa by androgen pathway.

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