Back

Widespread multi-targeted therapy resistance via drug-induced secretome fucosylation

Aldonza, M. B. D.; Cha, J.; Yong, I.; Ku, J.; Lee, D.; Sinitcyn, P.; Cho, R.-E.; Delos Reyes, R. D.; Kim, D.; Sung, H.-J.; Kim, S.; Kang, M.; Ku, Y.; Park, G.; Ryu, H. S.; Cho, S.; Kim, T. M.; Kim, P.; Cho, J.-Y.; Kim, Y.

2021-04-22 cancer biology
10.1101/2021.04.21.440719 bioRxiv
Show abstract

Cancer secretome is a reservoir for aberrant glycosylation. How therapies alter this post-translational cancer hallmark and the consequences thereof remain elusive. Here we show that an elevated secretome fucosylation is a pan-cancer signature of both response and resistance to multiple targeted therapies. Large-scale pharmacogenomics revealed that fucosylation genes display widespread association with resistance to these therapies. In both cancer cell cultures and patients, targeted kinase inhibitors distinctively induced core fucosylation of secreted proteins less than 60 kDa. Label-free proteomics of N-glycoproteomes revealed that fucosylation of the antioxidant PON1 is a critical component of the therapy-induced secretome. Core fucosylation in the Golgi impacts PON1 stability and folding prior to secretion, promoting a more degradation-resistant PON1. Non-specific and PON1-specific secretome de-N-glycosylation both limited the expansion of resistant clones in a tumor regression model. Our findings demonstrate that core fucosylation is a common modification indirectly induced by targeted therapies that paradoxically promotes resistance.

Matching journals

The top 7 journals account for 50% of the predicted probability mass.

50% of probability mass above

"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.