No observable guide-RNA-independent off-target mutation induced by prime editor
Gao, R.; Fu, Z.-C.; Li, X.; Wang, Y.; Wei, J.; Li, G.; Wang, L.; Wu, J.; Xue, W.; Huang, X.; Yang, L.; Chen, J.
Show abstract
Prime editor (PE) has been recently developed to induce efficient and precise ontarget editing, whereas its guide RNA (gRNA)-independent off-target effects remain unknown. Here, we used whole-genome and whole-transcriptome sequencing to determine gRNA-independent off-target mutations in cells expanded from single colonies, in which PE generated precise editing at on-target sites. We found that PE triggered no observable gRNA-independent off-target mutation genome-wide or transcriptome-wide in transfected human cells, highlighting its high specificity.
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