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A New Genus Of Microsporidian Parasite (Hepatosporidae; Microsporidia) Found In The Oocytes Of Ribbon Worms From The North Pacific Genus Maculaura (Heteronemertea; Nemertea)

Robbins, K. M.; Maslakova, S. A.; von Dassow, G.

2021-04-04 zoology
10.1101/2021.04.04.438387 bioRxiv
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An intracellular microsporidian parasite was first observed within oocytes of Maculaura alaskensis, a small pilidiophoran nemertean, commonly found on sandflats along the Pacific coast of North America. Infected oocytes have large vesicles containing dozens to hundreds of diplokaryotic, ellipsoid spores measuring 1.3 by 2.3 m. A partial small subunit nuclear ribosomal 18S gene sequence isolated from the microsporidian does not match any known microsporidian sequences in the public databases. Phylogenetic analysis groups it with Hepatospora eriocheir in a sister clade to the Enterocytozoonidae. All the known life stages of this parasite are contained within a membranous envelope. This microsporidian was identified in M. alaskensis, Maculaura aquilonia, Maculaura oregonensis, and Maculaura cerebrosa in Coos Bay, Oregon, in M. alaskensis from Newport, Oregon, and in M. aquilonia collected in Juneau, Alaska. This is, to our knowledge, the first species of microsporidia found to directly infect nemertean host cells. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=180 SRC="FIGDIR/small/438387v1_ufig1.gif" ALT="Figure 1"> View larger version (81K): org.highwire.dtl.DTLVardef@1b89feaorg.highwire.dtl.DTLVardef@aedc5org.highwire.dtl.DTLVardef@18030f2org.highwire.dtl.DTLVardef@10d7734_HPS_FORMAT_FIGEXP M_FIG C_FIG

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