Species and strain cultivation of skin, oral, and gut microbiota
Fleming, E.; Pabst, V.; Hoyt, A.; Zhou, W.; Hardy, R.; Peterson, A.; Beach, R.; Ondouah-Nzutchi, Y.; Dong, J.; Oh, J.
Show abstract
Genomics-driven discovery of microbial species have provided extraordinary insights into the biodiversity of human microbiota. High resolution genomics to investigate species- and strain-level diversity and mechanistic studies, however, rely on the availability of individual microbes from a complex microbial consortia. Here, we describe and validate a streamlined workflow for cultivating microbes from the skin, oral, and gut microbiota, informed by metagenomic sequencing, mass spectrometry, and strain profiling.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Preparation of functional metagenomic libraries from low biomass samples using METa assembly and their application to capture antibiotic resistance genes 96%
- Quantifying synthetic bacterial community composition with flow cytometry: efficacy in mock communities and challenges in co-cultures 95%
- Genomic characterization of the C. tuberculostearicum species complex, a ubiquitous member of the human skin microbiome 95%
Similar papers in this journal
- High-throughput single-cell isolation of Bifidobacterium strains from the gut microbiome 96%
- Microbial communities in retail draft beers and the biofilms they produce 95%
- Optimised methods for the targeted surveillance of extended-spectrum beta-lactamase producing Escherichia coli in human stool 95%
Similar papers in this journal
- Gut Microbiome Dynamics and Predictive Value in Hospitalized COVID-19 Patients: A Comparative Analysis of Shallow and Deep Shotgun Sequencing 95%
- Conserved organ-specific microbial assemblages in different populations of a terrestrial crab 94%
- Oropharyngeal Microbiome Profiled at Admission is Predictive of the Need for Respiratory Support Among COVID-19 Patients 94%
Similar papers in this journal
- Fusobacterium sphaericum sp. nov., isolated from a human colon tumor, adheres to colonic epithelial cells and induces IL-8 secretion 97%
- Addition of insoluble fiber to isolation media allows for increased metabolite diversity of lab-cultivable microbes derived from zebrafish gut samples 95%
- Actively replicating gut bacteria identified by 5-ethynyl-2'-deoxyuridine (EdU) click chemistry and cell sorting 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.