Spermiogenesis alterations in the absence of CTCF revealed by single cell RNA sequencing
Torres-Flores, U.; Diaz-Espinosa, F.; Lopez-Santaella, T.; Rebollar-Vega, R.; Vazquez-Jimenez, A.; Taylor, I. J.; Ortiz-Hernandez, R.; Echeverria, O. M.; Vazquez-Nin, G. H.; Gutierrez-Ruiz, M. C.; De la Rosa-Velazquez, I. A.; RESENDIS-ANTONIO, O.; Hernandez-Hernandez, A.
Show abstract
CTCF is an architectonical protein that organizes the genome inside the cell nucleus in almost all eukaryotic cells. There is evidence that CTCF plays a critical role during spermatogenesis as its depletion produces abnormal sperm and infertility. However, the defects produced by the absence of CTCF throughout spermatogenesis have not been characterized. In this work, we performed single cell RNA sequencing in spermatogenic cells without CTCF. We uncovered defects in transcriptional programs that explain the severity of the damage in the produced sperm. At early stages of spermatogenesis, transcriptional alterations are mild. As germ cells go throughout the specialization stage or spermiogenesis, transcriptional profiles become more altered. We found spermatid defects that support the alterations in the transcriptional profiles, and thus we conclude that CTCF depletion alters several transcriptional profiles mostly during spermiogenesis. Our data highlights the importance of CTCF at the different stages of spermatogenesis.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Topaz1, an essential gene for murine spermatogenesis, down-regulates the expression of numerous testis-specific long non-coding RNAs. 98%
- TAF4b Transcriptional Regulation During Cellular Quiescence of Developing Prospermatogonia 95%
- Transcriptomic Analysis of the Spatiotemporal Axis of Oogenesis and Fertilization in C. elegans 94%
Similar papers in this journal
- Phosphorylation of the Anaphase Promoting Complex activator CDH1/FZR regulates the transition from Meiosis I to Meiosis II in mouse male germ cell 95%
- The Golgi Glycoprotein MGAT4D is an Intrinsic Protector of Testicular Germ Cells From Mild Heat Stress 92%
- Short-term diet intervention alters the small non-coding RNA (sncRNA) landscape of human sperm 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.