Back

In vivo dissection of Rhoa function in vascular development using zebrafish

Pillay, L. M.; Yano, J. J.; Davis, A. E.; Butler, M. G.; Barnes, K. A.; Reyes, V. L.; Castranova, D.; Gore, A. V.; Swift, M. R.; Iben, J. R.; Stratman, A. N.; Weinstein, B. M.

2021-03-27 developmental biology
10.1101/2021.03.27.437282 bioRxiv
Show abstract

RationaleThe small monomeric GTPase RHOA acts as a master regulator of signal transduction cascades by activating effectors of cellular signaling, including the Rho-associated protein kinases ROCK1/2. Previous in vitro cell culture studies suggest that RHOA can regulate many critical aspects of vascular endothelial cell (EC) biology, including focal adhesion, stress fiber formation, and angiogenesis. However, the specific in vivo roles of RHOA during vascular development and homeostasis are still not well understood. ObjectiveIn this study we examine the in vivo functions of RHOA in regulating vascular development and integrity in zebrafish. Methods and ResultsWe use zebrafish RHOA-ortholog (rhoaa) mutants, transgenic embryos expressing wild type, dominant-negative, or constitutively active forms of rhoaa in ECs, and a pharmacologic inhibitor of ROCK1/2 to study the in vivo consequences of RHOA gain- and loss-of-function in the vascular endothelium. Our findings document roles for RHOA in vascular integrity, developmental angiogenesis, and vascular morphogenesis. ConclusionsOur results indicate that either too much or too little RHOA activity leads to vascular dysfunction in vivo.

Matching journals

The top 8 journals account for 50% of the predicted probability mass.

1
eLife
● 5828 papers in training set
Top 9%
11.4%
Similar papers in this journal
2
Development
● 497 papers in training set
Top 0.8%
10.2%
Similar papers in this journal
3
Developmental Dynamics
○ 56 papers in training set
Top 0.1%
7.0%
Similar papers in this journal
4
Blood Advances
● 62 papers in training set
Top 0.3%
6.0%
Similar papers in this journal
5
Nature Communications
○ 5641 papers in training set
Top 28%
5.2%
Similar papers in this journal
6
Scientific Reports
○ 3612 papers in training set
Top 18%
4.9%
7
Developmental Biology
○ 150 papers in training set
Top 0.5%
4.6%
8
Circulation Research
○ 47 papers in training set
Top 0.3%
4.2%
50% of probability mass above
9
Developmental Cell
○ 196 papers in training set
Top 0.9%
4.1%
10
Proceedings of the National Academy of Sciences
● 2444 papers in training set
Top 14%
3.9%
11
Cell Reports
○ 1498 papers in training set
Top 11%
3.3%
12
Cardiovascular Research
◐ 37 papers in training set
Top 0.4%
3.0%
13
Circulation
○ 74 papers in training set
Top 1%
2.6%
14
Disease Models & Mechanisms
139 papers in training set
Top 0.9%
2.3%
15
Frontiers in Cell and Developmental Biology
○ 233 papers in training set
Top 2%
1.8%
16
Molecular Biology of the Cell
● 311 papers in training set
Top 2%
1.8%
17
Arteriosclerosis, Thrombosis, and Vascular Biology
○ 71 papers in training set
Top 0.7%
1.8%
18
iScience
○ 1154 papers in training set
Top 22%
1.4%
19
Biology Open
● 156 papers in training set
Top 2%
1.4%
20
JCI Insight
● 277 papers in training set
Top 5%
1.3%
21
PLOS Biology
● 486 papers in training set
Top 8%
1.1%
22
Science Advances
● 1243 papers in training set
Top 27%
1.1%
23
PLOS ONE
● 5266 papers in training set
Top 57%
1.0%
24
The EMBO Journal
○ 309 papers in training set
Top 7%
0.8%
25
Angiogenesis
○ 14 papers in training set
Top 0.2%
0.8%
26
Journal of Cell Biology
● 392 papers in training set
Top 4%
0.8%
27
PLOS Genetics
● 862 papers in training set
Top 13%
0.8%
28
Communications Biology
○ 993 papers in training set
Top 32%
0.8%
29
The FASEB Journal
○ 194 papers in training set
Top 6%
0.8%
30
Life Science Alliance
● 285 papers in training set
Top 10%
0.6%

"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.