Isolation and Purification of Lipoarabinomannan from Urine of Adults with Active Tuberculosis
Cantera, J. L.; Rashid, A.; Lillis, L.; Peck, R.; Drain, P. K.; Pinter, A.; Kawasaki, M.; Moreau, E.; Boyle, D. S.
Show abstract
Lipoarabinomannan (LAM) is a cell wall component of Mycobacterium tuberculosis that is excreted in the urine of persons with active tuberculosis (TB). Limited diagnostic sensitivity of LAM immunoassays has been due to selecting antibodies against LAM derived from in vitro cultured M. tuberculosis, rather than LAM purified from in vivo clinical urine specimens. Urinary LAM (uLAM) is critical to enable the development of and/or screening of novel uLAM-specific antibodies but is typically dilute and in heterogeneous mixtures with other urine components. We used physical, enzymatic, and chemical processes for the scaled isolation and purification of uLAM. The purified material may then be used to develop more sensitive uLAM diagnostic tests for active TB disease.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Performance of novel antibodies for lipoarabinomannan to develop diagnostic tests for Mycobacterium tuberculosis 94%
- Development and validation of a highly sensitive and specific electrochemical assay to quantify anti-SARS-CoV-2 IgG antibodies to facilitate pandemic surveillance and monitoring of vaccine response 92%
- Evaluation of SARS-CoV-2 Antibody Point of Care Devices in the Laboratory and Clinical Setting 92%
Similar papers in this journal
Similar papers in this journal
- Comparison of LIAISON QuantiFERON-TB Gold Plus with QuantiFERON-TB Gold Plus 93%
- Bacterial growth-promoting properties of pooled urine and individual urines from post-menopausal women with or without a urinary tract infection can vary substantially 92%
- Analytical Validation of a Highly Accurate and Reliable Next-Generation Sequencing-Based Urine Assay 92%
Similar papers in this journal
- Protamine Protects Against Vancomycin Induced Kidney Injury 91%
- Glomerular function and urinary biomarker changes between vancomycin and vancomycin plus piperacillin-tazobactam in a translational rat model. 90%
- Impact of vancomycin loading doses and dose escalation on glomerular function and kidney injury biomarkers in a translational rat model 89%
Similar papers in this journal
- Small-molecule metabolome identifies potential therapeutic targets against COVID-19 91%
- Performance Comparison of a Flow Cytometry-based and Two Commercial Chemiluminescent Immunoassays for Detection and Quantification of Antibodies Binding to SARS-CoV-2 Spike Protein 91%
- Evaluation of the Access Bio CareStart™ rapid SARS-CoV-2 antigen test in asymptomatic individuals tested at a community mass-testing program in Western Massachusetts 90%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.