Development of a yeast-based assay for bioavailable phosphorous
Shepherd, H. A. M.; Trentman, M. T.; Tank, J. L.; Praner, J.; Cervantes, A.; Chaudhary, P.; Gezelter, J.; Marrs, A. J.; Myers, K. A.; Welsh, J. R.; Wu, Y.-F. O.; Goodson, H. V.
Show abstract
Preventing eutrophication of inland freshwater ecosystems requires quantifying the phosphorus (P) content of the streams and rivers that feed them. Typical methods for measuring P assess soluble reactive P (SRP) or total P (TP) and require expensive analytical techniques that produce hazardous waste. Here we present a novel method for measuring the more relevant bioavailable P (BAP); this assay utilizes the growth of familiar bakers yeast, avoids production of hazardous waste, and reduces cost relative to measurements of SRP and TP. The yeast BAP (yBAP) assay takes advantage of the observation that yeast density at saturating growth increases linearly with provided P. We show that this relationship can be used to measure P in freshwater in concentration ranges relevant to eutrophication. In addition, we measured yBAP in water containing known amounts of fertilizer and in samples from agricultural waterways. We observed that the majority of yBAP values were between those obtained from standard SRP and TP measurements, demonstrating that the assay is compatible with real-world settings. The cost-effective and nonhazardous nature of the yeast-based assay suggests that it could have utility in a range of settings, offering added insight to identify water systems at risk of eutrophication from excess phosphorus.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- In-field bioreactors demonstrate dynamic shifts in microbial communities in response to geochemical perturbations. 95%
- Participatory science methods to monitor water quality and ground truth remote sensing of the Chesapeake Bay 94%
- Composition Wheels: Visualizing dissolved organic matter using common composition metrics across a variety of Canadian Ecozones 94%
Similar papers in this journal
- Benchmarking concentration and direct extraction methods for wastewater-based surveillance of eight human respiratory viruses: implications for rapid application to novel pathogens 94%
- Solid evidence and liquid gold: trade-offs of processing settled solids, whole influent, or centrifuged influent for co-detecting viral, bacterial, and eukaryotic pathogens in wastewater 94%
- Detection of Hemagglutinin H5 influenza A virus RNA and model of potential inputs in an urban California sewershed 94%
Similar papers in this journal
- Persistence of endogenous SARS-CoV-2 and pepper mild mottle virus RNA in wastewater settled solids 95%
- Influence of Copper Dose on Mycobacterium avium and Legionella pneumophila Growth in Premise Plumbing 95%
- Within-Day Variability of SARS-CoV-2 RNA in Municipal Wastewater Influent During Periods of Varying COVID-19 Prevalence and Positivity 94%
Similar papers in this journal
- Detection and quantification of human immunodeficiency virus-1 (HIV-1) total nucleic acids in wastewater settled solids from two California communities 95%
- Development of wastewater pooled surveillance of SARS-CoV-2 from congregate living settings 95%
- Source-to-Tap Investigation of the Occurrence of Nontuberculous Mycobacteria in a Full-Scale Chloraminated Drinking Water System 94%
Similar papers in this journal
- Respiratory Syncytial Virus (RSV) RNA in wastewater settled solids reflects RSV clinical positivity rates 95%
- Regional replacement of SARS-CoV-2 variant BA.1 with BA.2 as observed through wastewater surveillance 94%
- Detection of hemagglutinin H5 influenza A virus sequence in municipal wastewater solids at wastewater treatment plants with increases in influenza A in spring, 2024 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.