A Phosphorylated Histone H2A Variant Displays Properties of Chromatin Insulator Proteins in Drosophila
Simmons, J. R.; An, R.; Amankwaa, B.; Zayac, S.; Kemp, J.; Labrador, M.
Show abstract
Chromatin insulators are responsible for mediating long-range interactions between enhancers and promoters throughout the genome and align with the boundaries of topologically associating domains (TADs). Here, we demonstrate an interaction between proteins that associate with the gypsy insulator and the phosphorylated histone variant H2Av ({gamma}H2Av), a marker of DNA double strand breaks. Gypsy insulator components colocalize with {gamma}H2Av throughout the genome. Mutation of insulator components prevents stable H2Av phosphorylation in polytene chromatin. Phosphatase inhibition strengthens the association between insulator components and {gamma}H2Av and rescues {gamma}H2Av localization in insulator mutants. We also show that {gamma}H2Av is a component of insulator bodies, and that phosphatase activity is required for insulator body dissolution after recovery from osmotic stress. We further demonstrate a tight association between {gamma}H2Av and TAD boundaries. Together, our results indicate a novel mechanism linking insulator function with a histone H2A variant and with genome stability.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Ambivalent partnership of the Drosophila posterior class Hox protein Abdominal-B with the Extradenticle and Homothorax cofactors 95%
- Cyclin B3 activates the Anaphase-Promoting Complex/Cyclosome in meiosis and mitosis 95%
- Fob1-dependent condensin recruitment and loop extrusion on yeast chromosome III 94%
Similar papers in this journal
- Tissue-specific regulation of translational readthrough tunes functions of the Traffic Jam transcription factor 94%
- The Synaptonemal Complex Central Region Modulates Crossover Pathways and Feedback Control of Meiotic Double-strand Break Formation 94%
- Bipartite recruitment of PCH2 and COMET to the synaptonemal complex drives chromosome axis reconstruction leading to crossover restriction 94%
Similar papers in this journal
Similar papers in this journal
- Dynamic neurotransmitter specific transcription factor expression profiles during Drosophila development 94%
- The Drosophila orthologue of the primary ciliary dyskinesia-associated gene, DNAAF3, is required for axonemal dynein assembly 94%
- Nucleolar stress in Drosophila neuroblasts, a model for human ribosomopathies 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.