Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro biofilm and in vivo implant infections
de Vor, L.; van Dijk, B.; van Kessel, K. P.; Kavanaugh, J. S.; de Haas, C. J.; Aerts, P. C.; Viveen, M. C.; Boel, E. C.; Fluit, A. C.; Kwiecinski, J. M.; Krijger, G. C.; Ramakers, R. M.; Beekman, F. J.; Dadachova, E.; Lam, M. G.; Vogely, H. C.; van der Wal, B. C.; van Strijp, J. A.; Horswill, A. R.; Weinans, H.; Rooijakkers, S. H.
Show abstract
Implant-associated Staphylococcus aureus infections are difficult to treat because of biofilm formation. Bacteria in a biofilm are often insensitive to antibiotics and host immunity. Monoclonal antibodies (mAbs) could provide an alternative approach to improve the diagnosis and/or treatment of biofilm-related infections. Here we show that mAbs targeting common surface components of S. aureus can recognize clinically relevant biofilm types. We identify two groups of antibodies: one group that uniquely binds S. aureus in biofilm state and one that recognizes S. aureus in both biofilm and planktonic state. In a mouse model, we show that mAb 4497 (recognizing wall teichoic acid (WTA)) specifically localizes to biofilm-infected implants. In conclusion, we demonstrate the capacity of several human mAbs to detect S. aureus biofilms in vitro and in vivo. This is an important first step to develop mAbs for imaging or treating S. aureus biofilms.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Comparative biofilm assays using Enterococcus faecalis OG1RF identify new determinants of biofilm formation 95%
- Fluoride export is required for competitive fitness of pathogenic microorganisms in dental biofilm models 94%
- Capsular polysaccharides cross-regulation modulates Bacteroides thetaiotaomicron biofilm formation 94%
Similar papers in this journal
- Identification of signaling pathways, matrix-digestion enzymes, and motility components controlling Vibrio cholerae biofilm dispersal 96%
- Architecture of cell-cell junctions in situ reveals a mechanism for bacterial biofilm inhibition 95%
- One gene, multiple ecological strategies: a biofilm regulator is a capacitor for sustainable diversity 95%
Similar papers in this journal
- Granuloma Dual RNA-Seq Reveals Composite Transcriptional Programs Driven by Neutrophils and Necrosis within Tuberculous Granulomas 93%
- The catheterized bladder environment promotes Efg1- and Als1-dependent Candida albicans infection 92%
- Emerging SARS-CoV-2 variants of concern evade humoral immune responses from infection and vaccination 92%
Similar papers in this journal
- Capsular polysaccharide inhibits vaccine-induced O-antigen antibody binding and function across both classical and hypervirulent K2:O1 strains of Klebsiella pneumoniae 94%
- MrvR, a group B Streptococcus transcription factor that controls multiple virulence traits 93%
- Unique growth and morphology properties of Clade 5 Clostridioides difficile strains revealed by single-cell time-lapse microscopy 93%
Similar papers in this journal
- Catalase Activity is Critical for Proteus mirabilis Biofilm Development, EPS Composition, and Dissemination During Catheter-Associated Urinary Tract Infection 95%
- Klebsiella pneumoniae L-Fucose metabolism promotes gastrointestinal colonization and modulates its virulence determinants 94%
- Parenteral Vaccination with recombinant EtpA glycoprotein impairs enterotoxigenic E. coli colonization 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.