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Detection Of Genomic Variants Of SARS-CoV-2 Circulating In Wastewater By High-Throughput Sequencing

Perez-Cataluna, A.; Chiner-Oms, A.; Cuevas-Ferrando, E.; Diaz-Reolid, A.; Falco, I.; Randazzo, W.; Giron-Guzman, I.; Allende, A.; Bracho, M. A.; Comas Espadas, I.; Sanchez, G.

2021-02-10 infectious diseases
10.1101/2021.02.08.21251355 medRxiv
Show abstract

The use of SARS-CoV-2 metagenomics in wastewater can allow the detection of variants circulating at community level. After comparing with clinical databases, we identified three novel variants in the spike gene, and six new variants in the spike detected for the first time in Spain. We finally support the hypothesis that this approach allows the identification of unknown SARS-CoV-2 variants or detected at only low frequencies in clinical genomes.

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