The pseudouridine synthase dyskerin binds to cytoplasmic H/ACA-box snoRNA retaining transcripts affecting nuclear hormone receptor dependence
Zacchini, F.; Venturi, G.; De Sanctis, V.; Bertorelli, R.; Ceccarelli, C.; Santini, D.; Taffurelli, M.; Penzo, M.; Trere, D.; Inga, A.; Dassi, E.; Montanaro, L.
Show abstract
Dyskerin is a nuclear protein involved in H/ACA box snoRNA-guided uridine modification of RNA. Since its defective function induces specific alterations in gene expression, we sought to unbiasedly identify mRNAs regulated by dyskerin. We found that dyskerin depletion affects the expression or the association with polysomes of selected mRNA isoforms characterized by the retention of H/ACA box snoRNA-containing introns. These snoRNA retaining transcripts (snoRTs) are bound by dyskerin and can interact with cytoplasmic ribosomes. We then characterized the cytoplasmic dyskerin RNA interactome finding both H/ACA box snoRTs and protein-coding transcripts. Since a fraction of these latter transcripts is involved in the nuclear hormone receptor binding, we tested to see if this specific activity is affected by dyskerin. Results indicate that dyskerin dysregulation may alter the dependence on nuclear hormone receptor ligands in breast cancer. Our work suggests a cytoplasmic function for dyskerin which could affect mRNA post-transcriptional networks relevant for nuclear hormone receptor functions.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- HELZ2: a new, interferon-regulated, human 3'-5' exoribonuclease of the RNB family is expressed from a non-canonical initiation codon 96%
- Human Prefoldin Modulates Co-transcriptional Pre-mRNA Splicing 96%
- Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification 96%
Similar papers in this journal
- Subcellular relocalization and nuclear redistribution of the RNA methyltransferases TRMT1 and TRMT1L upon neuronal activation 96%
- RNA Binding of GAPDH Controls Transcript Stability and Protein Translation in Acute Myeloid Leukemia 95%
- Xrn1 influence on gene transcription results from the combination of general effects on elongating RNA pol II and gene-specific chromatin configuration 93%
Similar papers in this journal
Similar papers in this journal
- Aurora-A phosphorylates splicing factors and regulates alternative splicing 95%
- A novel transcriptional signalling pathway mediated by the trafficking protein Ambra1 via scaffolding Atf2 complexes 94%
- Mapping the MOB proteins' proximity network reveals a unique interaction between human MOB3C and the RNase P complex 94%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.