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CRISPR Del/Rei: A simple, flexible and efficient pipeline for scarless genome editing

Wahbeh, M. H.; Feuer, K. L.; Abdollahi, S.; Yovo, C.; Rabie, E.; Lam, A.-T.; Young, L.; Avramopoulos, D.

2021-01-18 genetics
10.1101/2021.01.18.427163 bioRxiv
Show abstract

Scarless genome editing of induced pluripotent stem cells (iPSCs) is crucial for the precise modeling of genetic disease. Here we present CRISPR Del/Rei, a two-step deletion-reinsertion strategy with high editing efficiency and simple PCR-based screening that generates isogenic clones in ~2 months. We apply our strategy to edit iPSCs at 3 loci with only rare off target editing.

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