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Fibrin is a critical regulator of neutrophil effector function at mucosal barrier sites

Silva, L. M.; Doyle, A. D.; Tran, C. L.; Greenwell-Wild, T.; Dutzan, N.; Lum, A. G.; Agler, C. S.; Sibree, M.; Jani, P.; Martin, D.; Kram, V.; Castellino, F. J.; Flick, M. J.; Divaris, K.; Bugge, T. H.; Moutsopoulos, N. M.

2021-01-17 immunology
10.1101/2021.01.15.426743 bioRxiv
Show abstract

Tissue-specific cues are critical for homeostasis at mucosal barriers. Here, we document that the clotting factor fibrin is a critical regulator of neutrophil function at mucosal barriers. We demonstrate that fibrin engages neutrophils through the M{beta}2 integrin receptor and activates effector functions, including the production of reactive oxygen species and NET formation. These immune-protective neutrophil functions become tissue damaging in the context of impaired plasmin-mediated fibrinolysis. Indeed, the accumulation of fibrin due to Mendelian genetic defects in plasmin leads to severe oral mucosal immunopathology in mice and humans. Concordantly, genetic polymorphisms in the human PLG gene, encoding plasminogen, are associated with common forms of the oral mucosal disease periodontitis. Our work uncovers fibrin as a critical regulator of neutrophil effector function within the mucosal tissue microenvironment and suggests fibrin-neutrophil engagement as a pathogenic instigator and therapeutic target in common mucosal disease.

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