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Large-scale cellular-resolution imaging of neural activity in freely behaving mice

Leman, D. P.; Chen, I. A.; Bolding, K. A.; Tai, J.; Wilmerding, L. K.; Gritton, H. J.; Cohen, Y.; Yen, W. W.; Perkins, L. N.; Liberti, W. A.; Kilic, K.; Han, X.; Cruz-Martin, A.; Gardner, T. J.; Otchy, T. M.; Davison, I. G.

2021-01-17 neuroscience
10.1101/2021.01.15.426462 bioRxiv
Show abstract

Miniaturized microscopes for head-mounted fluorescence imaging are powerful tools for visualizing neural activity during naturalistic behaviors, but the restricted field of view of first-generation miniscopes limits the size of neural populations accessible for imaging. Here we describe a novel miniaturized mesoscope offering cellular-resolution imaging over areas spanning several millimeters in freely moving mice. This system enables comprehensive visualization of activity across entire brain regions or interactions across areas.

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