Strong and tunable anti-CRISPR/Cas9 activity of AcrIIA4 in plants
Calvache, C.; Vazquez-Vilar, M.; Selma, S.; Uranga, M.; Daros, J. A.; Orzaez, D.
Show abstract
This study describes the strong anti-CRISPR activity of the bacterial AcrIIA4 protein in Nicotiana benthamiana, a model plant used as molecular farming platform. The results demonstrate that AcrIIA4 abolishes site-directed mutagenesis in leaves when transiently co-expressed with CRISPR/Cas9. We also show that AcrIIA4 represses CRISPR/dCas9-based transcriptional activation (CRISPRa) of both reporter and endogenous genes in a highly efficient, dose-dependent manner. Furthermore, the fusion of an auxin degron to AcrIIA4 results in auxin-regulated activation of a downstream reporter gene. The strong anti-Cas9 activity of AcrIIA4 reported here opens new possibilities for customized control of gene editing and gene expression in plants.
Matching journals
The top 3 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- High performance TadA-8e derived cytosine and dual base editors with undetectable off-target effects in plants 95%
- Phytochrome-dependent responsiveness to root-derived cytokinins enables coordinated elongation responses to combined light and nitrate cues. 95%
- COMPOSITUM 1 (COM1) contributes to the architectural simplification of barley inflorescence via meristem identity signals 95%
Similar papers in this journal
Similar papers in this journal
- A quantitative autonomous bioluminescence reporter system with a wide dynamic range for Plant Synthetic Biology 97%
- CuBe: a geminivirus-based copper-regulated expression system suitable for post-harvest activation. 94%
- A chromosome-scale assembly of allotetraploid Brassica juncea (AABB) elucidates comparative architecture of the A and B genomes 94%
Similar papers in this journal
- Genome-wide identification and characterization of Solanum tuberosum BiP genes reveals the role of the promoter architecture in BiP gene diversity 94%
- Apoplastic effector candidates of a foliar forest pathogen trigger cell death in host and non-host plants 93%
- Transcriptional signatures of wheat inflorescence development 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.