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GC-1 spg cells are most similar to Leydig cells, a testis somatic cell-type, and not germ cells

Norman, A. R.; Byrnes, L.; Reiter, J. R.

2021-01-07 cell biology
10.1101/2021.01.07.425754 bioRxiv
Show abstract

GC-1 spg is an immortalized cell line derived from an adult mouse testis and reported to be most similar to spermatocytes, a male germ cell-type. However, immunofluorescence indicates that GC-1 spg cells express WT1, a marker of testis somatic cells, and do not express markers of germ cells. Transcriptomic profiling indicate GC-1 cells are most similar to Leydig cells. Therefore, we conclude that GC-1 spg cells are most similar to testis somatic cells.

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