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Mechanical unloading is associated with decreased DNA content in cardiomyocytes independent of nucleation state.

2020-12-22 cardiovascular medicine Title + abstract only
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BackgroundCardiomyocytes increase DNA content in response to stress in humans. Proliferation has been reported in cardiomyocytes in failing hearts and following LVAD unloading which may represent a resolution of this process through cell division. However, cardiac recovery from LVAD is rare. MethodsWe quantified cardiomyocyte nuclear number, cell size, DNA content and the frequency of cell cycling markers by imaging flow cytometry from human subjects undergoing LVAD implantation or primary tran...

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