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Cytosplore-Transcriptomics: a scalable inter-active framework for single-cell RNA sequenc-ing data analysis

Abdelaal, T.; Eggermont, J.; Hollt, T.; Mahfouz, A.; Reinders, M.; Lelieveldt, B.

2020-12-12 bioinformatics
10.1101/2020.12.11.421883 bioRxiv
Show abstract

The ever-increasing number of analyzed cells in Single-cell RNA sequencing (scRNA-seq) experiments imposes several challenges on the data analysis. Current analysis methods lack scalability to large datasets hampering interactive visual exploration of the data. We present Cytosplore-Transcriptomics, a framework to analyze scRNA-seq data, including data preprocessing, visualization and downstream analysis. At its core, it uses a hierarchical, manifold preserving representation of the data that allows the inspection and annotation of scRNA-seq data at different levels of detail. Consequently, Cytosplore-Transcriptomics provides interactive analysis of the data using low-dimensional visualizations that scales to millions of cells. AvailabilityCytosplore-Transcriptomics can be freely downloaded from transcriptomics.cytosplore.org Contactb.p.f.lelieveldt@lumc.nl

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