IGI-LuNER: single-well multiplexed RT-qPCR test for SARS-CoV-2
Stahl, E. C.; Tsuchida, C. A.; Hamilton, J. R.; Lin-Shiao, E.; McDevitt, S. L.; Moehle, E. A.; Witkowsky, L. B.; Tsui, C. K.; Pestal, K.; Gildea, H. K.; McElroy, M.; Keller, A.; Sylvain, I.; Williams, C.; Hirsh, A.; Ciling, A.; Ehrenberg, A. J.; SARS-CoV-2 consortium, ; Urnov, F. D.; Ringeisen, B. R.; Giannikopoulos, P.; Doudna, J. A.
Show abstract
Commonly used RT-qPCR-based SARS-CoV-2 diagnostics require 2-3 separate reactions or rely on detection of a single viral target, adding time and cost or risk of false-negative results. Currently, no test combines detection of widely used SARS-CoV-2 E- and N-gene targets and a sample control in a single, multiplexed reaction. We developed the IGI-LuNER RT-qPCR assay using the Luna Probe Universal One-Step RT-qPCR master mix with publicly available primers and probes to detect SARS-CoV-2 N gene, E gene, and human RNase P (NER). This combined, cost-effective test can be performed in 384-well plates with detection sensitivity suitable for clinical reporting, and will aid in future sample pooling efforts, thus improving throughput of SARS-CoV-2 detection. Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=79 SRC="FIGDIR/small/20247338v2_ufig1.gif" ALT="Figure 1"> View larger version (27K): org.highwire.dtl.DTLVardef@74929corg.highwire.dtl.DTLVardef@1457971org.highwire.dtl.DTLVardef@2825ddorg.highwire.dtl.DTLVardef@1cde2b6_HPS_FORMAT_FIGEXP M_FIG C_FIG
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