DisA limits RecA- and RadA/Sms-mediated replication fork remodelling to prevent genome instability
Torres, R.; Alonso, J. C.
Show abstract
The DisA diadenylate cyclase (DAC), the DNA helicase RadA/Sms and the RecA recombinase are required to prevent a DNA replication stress during the revival of haploid Bacillus subtilis spores. Moreover, disA, radA and recA are epistatic among them in response to DNA damage. We show that DisA inhibits the ATPase activity of RadA/Sms C13A by competing for single-stranded (ss) DNA. In addition, DisA inhibits the helicase activity of RadA/Sms. RecA filamented onto ssDNA interacts with and recruits DisA and RadA/Sms onto branched DNA intermediates. In fact, RecA binds a reversed fork and facilitates RadA/Sms-mediated unwinding to restore a 3'-fork intermediate, but DisA inhibits it. Finally, RadA/Sms inhibits DisA DAC activity, but RecA counters this negative effect. We propose that RecA, DisA and RadA/Sms interactions, which are mutually exclusive, limit remodelling of stalled replication forks. DisA, in concert with RecA and/or RadA/Sms, indirectly contributes to template switching or lesion bypass, prevents fork breakage and facilitates the recovery of c-di-AMP levels to re-initiate cell proliferation. Subject CategoriesGenomic stability & Dynamics
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Intrinsically disordered regions regulate RhlE RNA helicase functions in bacteria 94%
- POT-3 preferentially binds the terminal DNA-repeat on the telomeric G-overhang 94%
- Cas3 Mediated Target DNA Recognition and Cleavage is Independent of the Composition and Architecture of Cascade Surveillance Complex 94%
Similar papers in this journal
- The Cdc14 phosphatase controls resolution of recombination intermediates and crossover formation during meiosis 93%
- Two XMAP215/TOG microtubule polymerases, Alp14 and Dis1, play non-exchangeable, distinct roles in microtubule organisation in fission yeast 92%
- Cracking the floral quartet code: How do multimers of MIKC C-type MADS-domain transcription factors recognize their target genes? 92%
Similar papers in this journal
- Consequences of alanine-126 mutations in helix-3 on structure and functions of Rad6 E2 ubiquitin-conjugating enzymes 94%
- DNA ligase I fidelity mediates the mutagenic ligation of pol β oxidized nucleotide insertion products and base excision repair intermediates with mismatches 93%
- Dimerization of Cdc13 is essential for dynamic DNA exchange on telomeric DNA 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.