Clonal expansion of personalized anti-tumor T cells from circulation using tumor organoid-immune co-cultures
Meng, Q.; Xie, S.; Gray, K.; Li, W.; Huang, L.; Akshinthala, D.; Dezfulian, M.; Ferrer, E.; Conahan, C.; Del Pino, S.; Grossman, J.; Elledge, S.; Hidalgo, M.; Muthuswamy, S. K.
Show abstract
Tumor-specific cytotoxic T cells are effective tools for cancer immunotherapy, but the ability to generate them continues to be a challenge. Furthermore, there are no compelling approaches to empirically identify tumor-targeting T cells and T cell receptors by exploiting the multitude of antigens on tumor cell surfaces. Here, we use patients peripheral blood and autologous tumor organoids to enrich tumor-specific cytotoxic T cells with patient-specific killing mechanisms and a tissue-resident memory phenotype. We further demonstrate that these organoid-primed T (opT) cells undergo several orders of magnitude of clonal expansion and express T cells receptors and check-point proteins unique to each patient. Importantly, transferring the TCRs to heterologous T cells was sufficient to confer tumor recognition in a patient-specific manner. Thus, we report a patient-specific and antigen-agnostic platform for expansion of tumor-targeting T cells and identification of cancer-targeting TCRs from the peripheral blood of pancreatic cancer patients that can be exploited for immunotherapy applications.
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