Binding site localization on non-homogeneous cell surfaces using topological image averaging
Kumra Ahnlide, V.; Kumra Ahnlide, J.; Beech, J. P.; Nordenfelt, P.
Show abstract
Antibody binding to cell surface proteins plays a crucial role in immunity and the location of an epitope can altogether determine the immunological outcome of a host-target interaction. Techniques available today for epitope identification are costly, time-consuming, and unsuited for high-throughput analysis. Fast and efficient screening of epitope location can be useful for the development of therapeutic monoclonal antibodies and vaccines. In the present work, we have developed a method for imaging-based localization of binding sites on cellular surface proteins. The cellular morphology typically varies, and antibodies often bind in a non-homogenous manner, making traditional particle-averaging strategies challenging for accurate native antibody localization. Nanometer-scale resolution is achieved through localization in one dimension, namely the distance from a bound ligand to a reference surface, by using topological image averaging. Our results show that this method is well suited for antibody binding site measurements on native cell surface morphology.
Matching journals
The top 9 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Benchmarking of novel green fluorescent proteins for the quantification of protein oligomerization in living cells 93%
- A framework to enhance the Signal-to-Noise Ratio for quantitative fluorescence microscopy 92%
- Fluorescent reporter plasmids for single-cell and bulk-level composition assays in E. faecalis 91%
Similar papers in this journal
- An evaluation of multi-excitation-wavelength standing-wave fluorescence microscopy (TartanSW) to improve sampling density in studies of the cell membrane and cytoskeleton 92%
- ColTapp, an automated image analysis application for efficient microbial colony growth dynamics quantification 92%
- Generating kinetic environments to study dynamic cellular processes in single cells 91%
Similar papers in this journal
Similar papers in this journal
- Photochromic fluorophores enable imaging of lowly-expressed proteins in the autofluorescent fungus Candida albicans 94%
- Three-dimensional observations of an aperiodic oscillatory gliding motility behaviour in Myxococcus xanthus using confocal interference reflection microscopy 93%
- The Dynamics of Cryptococcus neoformans infection in Galleria mellonella 91%
Similar papers in this journal
- pHusion: A robust and versatile toolset for automated detection and analysis of exocytosis 91%
- VISION -- an open-source software for automated multi-dimensional image analysis of cellular biophysics 91%
- High-speed multifocal plane fluorescence microscopy for three-dimensional visualisation of beating flagella 91%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.