High-throughput phenotypic characterization of zebrafish CRISPR mutants of DNA repair genes
Shin, U.; Nakhro, K.; Oh, C.-K.; Carrington, B.; Song, H.; Varshney, G. K.; Kim, Y.; Song, H.; Jeon, S.; Robbins, G.; Kim, S.; Yoon, S.; Choi, Y.; Park, S.; Kim, Y. J.; Burgess, S. M.; Kang, S.; Sood, R.; Lee, Y.; Myung, K.
Show abstract
A systematic knowledge of the roles of DNA repair genes at the level of the organism has been limited due to the lack of appropriate experimental techniques. Here, we generated zebrafish loss-of-function mutants for 32 DNA repair and replication genes through multiplexed CRISPR/Cas9-mediated mutagenesis. High-throughput phenotypic characterization of our mutant collection revealed that three genes (atad5a, ddb1, pcna) are essential for proper embryonic development and hematopoiesis; seven genes (apex1, atrip, ino80, mre11a, shfm1, telo2, wrn) are required for growth and development during juvenile stage and six genes (blm, brca2, fanci, rad51, rad54l, rtel1) play critical roles in sex development. Furthermore, mutation in six genes (atad5a, brca2, polk, rad51, shfm1, xrcc1) displayed hypersensitivity to DNA damage agents. Further characterization of atad5a-/- mutants demonstrate that Atad5a is required for normal brain development and hematopoiesis. Our zebrafish mutant collection provides a unique resource for understanding of the roles of DNA repair genes at the organismal level.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Banp regulates DNA damage response and chromosome segregation during the cell cycle in zebrafish retina 96%
- Lin28a/let-7 Pathway Modulates the Hox Code via Polycomb Regulation during Axial Patterning in Vertebrates 95%
- Oncogenic and teratogenic effects of Trp53Y217C, an inflammation-prone mouse model of the human hotspot mutant TP53Y220C 95%
Similar papers in this journal
- Variability of an early developmental cell population underlies stochastic laterality defects 94%
- Piezo1-dependent regulation of pericyte proliferation by blood flow during brain vascular development 93%
- Fragile X Mental Retardation Protein regulates R-loop formation and prevents global chromosome fragility 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.