Back

Self-Organizing 3D Human Choroid Plexus-Ventricle-Cortical Organoids

Shaker, M. R.; Cooper-White, J.; Wolvetang, E. J.

2020-10-01 neuroscience
10.1101/2020.09.30.321554 bioRxiv
Show abstract

Both the choroid plexus (CP) and the cortex are derived from the rostral neural tube during early embryonic development. In addition to producing CSF, the CP secretes essential factors that orchestrate cortical development and later neurogenesis. Previous brain modeling efforts with human pluripotent stem cells (hPSCs) generated either cortical or CP tissues in 3D culture. Here, we used hPSC-derived neuroectodermal cells, the building blocks of the anterior body, to simultaneously generate CP that forms ventricles and cortical cells in organoids (CVCOs), which can be maintained as 3D organoid cultures. Large scale culture revealed reproducibility of the protocol independent of cell lines, clones or batches. CVCOs contain mature and functional CP that projects multiple cilia into the ventricle-like fluid filled cysts and is in direct contact with appropriately patterned cortical cells. CVCOs thus recapitulate key features of developing forebrain structures observed in in vivo and constitute a useful for dissecting the role of CP in human forebrain development in health and disease.

Matching journals

The top 5 journals account for 50% of the predicted probability mass.

1
eLife
● 5828 papers in training set
Top 2%
22.2%
Similar papers in this journal
2
Disease Models & Mechanisms
139 papers in training set
Top 0.1%
11.8%
3
Development
● 497 papers in training set
Top 1.0%
7.8%
Similar papers in this journal
4
iScience
○ 1154 papers in training set
Top 2%
5.4%
Similar papers in this journal
5
Stem Cell Reports
○ 130 papers in training set
Top 0.4%
5.4%
Similar papers in this journal
50% of probability mass above
6
Cell Stem Cell
○ 62 papers in training set
Top 0.3%
5.1%
7
Cell Reports
○ 1498 papers in training set
Top 10%
4.0%
8
EMBO Reports
○ 263 papers in training set
Top 2%
2.4%
9
Cells
○ 249 papers in training set
Top 2%
2.4%
10
eneuro
● 439 papers in training set
Top 3%
2.4%
11
Developmental Cell
○ 196 papers in training set
Top 2%
2.1%
12
Communications Biology
○ 993 papers in training set
Top 12%
1.9%
13
Nature Communications
○ 5641 papers in training set
Top 45%
1.7%
14
Scientific Reports
○ 3612 papers in training set
Top 52%
1.7%
15
PLOS Biology
● 486 papers in training set
Top 5%
1.7%
16
Frontiers in Molecular Neuroscience
○ 47 papers in training set
Top 0.6%
1.5%
17
Life Science Alliance
● 285 papers in training set
Top 4%
1.4%
18
Proceedings of the National Academy of Sciences
● 2444 papers in training set
Top 35%
1.1%
19
Frontiers in Cell and Developmental Biology
○ 233 papers in training set
Top 4%
1.1%
20
Frontiers in Neuroscience
○ 256 papers in training set
Top 5%
1.0%
21
Current Biology
○ 665 papers in training set
Top 9%
0.9%
22
Science Advances
● 1243 papers in training set
Top 31%
0.8%
23
Glia
○ 81 papers in training set
Top 1%
0.8%
24
EMBO Molecular Medicine
○ 95 papers in training set
Top 3%
0.8%
25
JCI Insight
● 277 papers in training set
Top 8%
0.8%
26
Open Biology
● 106 papers in training set
Top 2%
0.8%
27
Stem Cell Research
○ 16 papers in training set
Top 0.2%
0.8%
28
Journal of Cell Science
● 393 papers in training set
Top 5%
0.6%

"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.