TransMPRA: A framework for assaying the role of many trans-acting factors at many enhancers
Calderon, D.; Ellis, A.; Daza, R.; Martin, B.; Tome, J.; Chen, W.; Chardon, F.; Leith, A.; Lee, C.; Martin, B.; Trapnell, C.; Shendure, J.
Show abstract
Gene regulation occurs through trans-acting factors (e.g. transcription factors) acting on cis-regulatory elements (e.g. enhancers). Massively parallel reporter assays (MPRAs) functionally survey large numbers of cis-regulatory elements for regulatory potential, but do not identify the trans-acting factors that mediate any observed effects. Here we describe transMPRA -- a reporter assay that efficiently combines multiplex CRISPR-mediated perturbation and MPRAs to identify trans-acting factors that modulate the regulatory activity of specific enhancers.
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