mir-218 modulates ARF6 expression and inhibits pancreatic ductal adenocarcinoma invasion
Rheinheimer, B.; Heimark, R. L.
Show abstract
BackgroundPancreatic ductal adenocarcinoma is an extremely malignant disease with the majority of patients having metastatic disease upon diagnosis. Recently, it was shown the SLIT2 plays a regulatory role in melanoma invasion through the control of invadopodia. Therefore, we sought to determine the mechanism behind miR-218 inhibition of pancreatic cancer invasion. Methodsmir-218 target genes were discovered using three miRNA target prediction websites. Modulation of mir-218 target ARF6 was determined by transfection with antagomirs and mimics to mir-218. Pancreatic cancer migration and invasion were measured using Boyden chamber assays. Invasion was further measured using matrix degradation assays. ResultsWe found that mir-218 modulates ARF6 RNA and protein expression in pancreatic cancer. mir-218 did not inhibit pancreatic cancer cell migration, but did inhibit invasion. mir-218 did not affect the formation of invadopodia in pancreatic cancer cells, but did inhibit the total area of matrix degradation caused by functional invadopodia. ConclusionsThis work suggests that miR-218 is a suppressor of pancreatic ductal adenocarcinoma invasion through a pathway that regulates invadopodia maturation.
Matching journals
The top 9 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- IL-1-conferred gene expression pattern in ERα+ BCa and AR+ PCa cells is intrinsic to ERα- BCa and AR- PCa cells and promotes cell survival 93%
- Identification of novel exosomal miRNAs and their role in diagnosis and prognosis of Triple Negative Breast Cancer 92%
- GDNF-RET signaling and EGR1 form a positive feedback loop that promotes tamoxifen resistance via cyclin D1 92%
Similar papers in this journal
- Altering mammalian transcription networking with ADAADi: An inhibitor of ATP-dependent chromatin remodeling 93%
- Oncoprotein 18 is necessary for malignant cell proliferation in bladder cancer cells and serves as a G3-specific non-invasive diagnostic marker candidate in urinary RNA 92%
- Chronic IL-1 exposure drives LNCaP cells to evolve androgen and AR independence 91%
Similar papers in this journal
- The TLK1-MK5 axis regulates motility, invasion, and metastasis of prostate cancer cells 93%
- Heparan-6-O-endosulfatase 2 promotes invasiveness of head and neck squamous carcinoma cell lines in co-cultures with cancer associated fibroblasts 93%
- Suppression of Ovarian Cancer Cell Proliferation is Associated with Upregulation of Cell-Matrix Adhesion Programs and Integrin-β4-Induced Cell Protection from Cisplatin. 92%
Similar papers in this journal
- Identifying and targeting key driver genes for collagen production within the 11q13/14 breast cancer amplicon 92%
- The target gene regulatory network of miR-497 in angiosarcoma 92%
- Disrupting β-catenin dependent Wnt signaling activates an invasive gene program predictive of colon cancer progression 91%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.