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Enhanced tissue penetration of antibodies through pressurized immunohistochemistry

Fiorelli, R.; Sidhu, G. S.; Cebrian-Silla, A.; Luna Melendez, E.; Mehta, S.; Garcia-Verdugo, J. M.; Sanai, N.

2020-09-28 molecular biology
10.1101/2020.09.25.311936 bioRxiv
Show abstract

To address the inefficiency of passive diffusion for antibody penetration in thick tissue samples, which limits clearing-technique applications, we developed a versatile and simple device to perform antibody incubation under increased barometric pressure. Pressurized immunohistochemistry greatly improves the uniformity, intensity, and depth of fluorescent immunostaining in thick human and mouse brain samples. Furthermore, pressurized immunohistochemistry substantially decreases the time required for classic staining of thin sections. SUBMISSION CATEGORYNew Results

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.