Metazoan-like kinetochore arrangement masked by the interphase RabI configuration
Jimenez-Martin, A.; Pineda-Santaella, A.; Leon-Perinan, D.; Delgado-Gestoso, D.; Marin-Toral, L.; Fernandez-Alvarez, A.
Show abstract
During cell cycle progression in metazoan, the kinetochore, the protein complex attached to centromeres which directly interacts with the spindle microtubules, the vehicle of chromosome segregation, is assembled at mitotic onset and disassembled during mitotic exit. This program is assumed to be absent in budding and fission yeast because kinetochore proteins are stably maintained at the centromeres throughout the entire cell cycle. In this work, we show that the assembly program at the mitotic onset of the Ndc80 complex, a crucial part of the outer kinetochore, is unexpectedly conserved in Schizosaccharomyces pombe. We have identified this behavior by removing the Rabl chromosome configuration during interphase, in which centromeres are permanently associated with the nuclear envelope beneath the spindle pole body. Hence, the Rabl configuration masks the presence of a program to recruit Ndc80 at mitotic onset in fission yeast, similar to that taking place in metazoan. Besides the evolutionary implications of our observations, we think that our work will help understand the molecular processes behind the kinetochore assembly program during mitotic entry using fission yeast as the model organism.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Cik1 and Vik1 Accessory Proteins Confer Distinct Functions to the Kinesin-14, Kar3 95%
- Mitotic chromosome condensation requires phosphorylation of the centromeric protein KNL-2 in C. elegans 95%
- S. pombe DNA translocases Rrp1 and Rrp2 have distinct roles at centromeres and telomeres that ensure genome stability. 95%
Similar papers in this journal
- Aspergillus SUMOylation mutants have normal dynein function but exhibit chromatin bridges 96%
- The Polo kinase Cdc5 is regulated at multiple levels in the adaptation response to telomere dysfunction 95%
- A non-canonical Hippo pathway regulates spindle disassembly and cytokinesis during meiosis in Saccharomyces cerevisiae 95%
Similar papers in this journal
- Distinct Aurora B pools at the inner centromere and kinetochore have different contributions to meiotic and mitotic chromosome segregation 95%
- Mps1 promotes poleward chromosome movements in meiotic pro-metaphase 95%
- Redistribution of centrosomal proteins by centromeres and Polo kinase controls partial nuclear envelope breakdown in fission yeast 95%
Similar papers in this journal
Similar papers in this journal
- Phosphorylation of luminal region of the SUN-domain protein Mps3 promotes nuclear envelope localization during meiosis 95%
- Cohesin reconstitution and homologous recombination repair of DNA double strand breaks in late mitosis 95%
- Control of telomere length in yeast by SUMOylated PCNA and the Elg1 PCNA unloader 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.