Proteo-transcriptomic profiling of nasal mononuclear phagocytic system cells in human controlled allergen challenge
Voskamp, A. L.; Gerdes, M. L.; Menafra, R.; Duijster, E.; Kielbasa, S. M.; Groot Kormelink, T.; Tak, T.; Stam, K. A.; de Jong, N. W.; Hendricks, R. W.; Kloet, S. L.; Yazdanbakhsh, M.; De Jong, E. C.; Gerth van Wijk, R.; Smits, H. H.
Show abstract
Innate mononuclear phagocytic system (MPS) cells preserve mucosal immune homeostasis. Here, we investigated their role at nasal mucosa following challenge with house dust mite. We combined single cell proteome and transcriptome profiling on immune cells from nasal biopsy cells of allergic rhinitis and non-allergic subjects, before and after repeated nasal allergen challenge. Nasal biopsies of patients showed infiltrating inflammatory HLA-DRhi CD14+ monocytes and CD16+ monocytes, and transcriptional changes in resident CD1C+ CD1A+ conventional dendritic cells (cDC)2 following challenge. Importantly, although clinically silent, non-allergic individuals displayed a distinct innate MPS response to allergen challenge: predominant infiltration of myeloid-derived suppressor cells (HLA-DRlow CD14+ monocytes), as well as cDC2 clusters expressing increased inhibitory/tolerogenic transcripts. Therefore, we identified not only clusters involved in airway inflammation but also a non-inflammatory, homeostatic blueprint of innate MPS responses to allergens in non-allergic individuals. Future therapies should target innate MPS for treatment of inflammatory airway diseases. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=112 SRC="FIGDIR/small/20189886v2_ufig1.gif" ALT="Figure 1"> View larger version (39K): org.highwire.dtl.DTLVardef@e6e970org.highwire.dtl.DTLVardef@1e7e8bdorg.highwire.dtl.DTLVardef@60d55forg.highwire.dtl.DTLVardef@1587d4d_HPS_FORMAT_FIGEXP M_FIG C_FIG
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