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Tet2 Controls Beta cells Responses to Inflammation in Type 1 Diabetes

Rui, J.; Deng, S.; Ponath, G.; Kursawe, R.; Lawlor, N.; Sumida, T.; Levine-Ritterman, M.; Perdigoto, A. L.; Stitzel, M. L.; Pitt, D. L.; Lu, J.; Herold, K. C.

2020-09-02 immunology
10.1101/2020.09.01.278028 bioRxiv
Show abstract

{beta} cells may participate and contribute to their own demise during Type 1 diabetes (T1D). We identified a novel role of Tet2 in regulating immune killing of {beta} cells. Tet2 is induced in murine and human {beta} cells with inflammation but its expression is reduced in surviving {beta} cells. Tet2-KO mice that receive WT bone marrow transplants develop insulitis but not diabetes and islet infiltrates do not eliminate {beta} cells even though immune cells from the mice can transfer diabetes to NOD/scid recipients. Tet2-KO {beta} cells show reduced expression of inflammatory genes, associated with closed transcription factor binding sites. Tet2-KO recipients are protected from transfer of disease by diabetogenic immune cells. We conclude that Tet2 regulates pathologic interactions between {beta} cells and immune cells and controls intrinsic protective pathways. Modulating TET2 may enable survival of {beta} cells or their replacements in the setting of pathologic immune cells.

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