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Microbial activation converts neutrophils into anti-tumor effectors

Yam, A. O.; Bailey, J.; Lin, F.; Jakovija, A.; Counoupas, C.; Triccas, J. A.; Gunzer, M.; Bald, T.; Grey, S. T.; Chtanova, T.

2020-08-23 immunology
10.1101/2020.08.21.259051 bioRxiv
Show abstract

Neutrophils infiltrate most solid tumors and their presence is usually correlated with suppression of anti-tumor responses, metastasis and poor prognosis. Here we used microbial bioparticles administered into the tumor microenvironment to transform neutrophils into anti-tumor effectors. Microbially activated neutrophils acquired an effector phenotype associated with pathogen killing and lost vascular endothelial growth factor expression associated with tumor growth and metastasis. They became the dominant immune cell infiltrating the tumor and inhibited tumor growth. Using intravital two-photon microscopy microbially activated neutrophils could be seen forming close contacts with tumor cells resulting in tumor tissue remodeling and tumor cell death. Thus, microbial bioparticle treatment can endow neutrophils with anti-tumor properties, suggesting that neutrophil plasticity in cancer could be exploited for tumor killing. These data highlight a pathway for the rational development of neutrophil-based cancer therapy.

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