Super-resolution light-sheet fluorescence microscopy by SOFI.
Mizrachi, J.; Narasimhan, A.; Qi, X.; Drewes, R.; Palaniswamy, R.; Wu, Z.; Osten, P.
Show abstract
Here we describe a new method, named LS-SOFI, that combines light-sheet fluorescence microscopy and super-resolution optical fluctuation imaging to achieve fast nanoscale-resolution imaging over large fields of view in native 3D tissues. We demonstrate the use of LS-SOFI in super-resolution analysis of neuronal structures and synaptic proteins, including cortical axons, dendritic spines, pre- and postsynaptic cytoskeletal proteins and postsynaptic AMPA receptors, in thick mouse brain sections. We also introduce an algorithm to determine the number of active fluorophore emitters detected, allowing the localization of individual molecules in LS-SOFI images. We conclude that LS-SOFI is a versatile method for fast super-resolution imaging from any tissue of the body using both commercial and custom LSFM instruments.
Matching journals
The top 2 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Minutes-timescale 3D isotropic imaging of entire organs at subcellular resolution by content-aware compressed-sensing light-sheet microscopy 97%
- Photon-free (s)CMOS camera characterization for artifact reduction in high- and super-resolution microscopy 97%
- Direct Supercritical Angle Localization Microscopy for Nanometer 3D Superresolution 96%
Similar papers in this journal
- A spectral demixing method for high-precision multi-color localization microscopy 97%
- Fast volumetric fluorescence lifetime imaging of multicellular systems using single-objective light-sheet microscopy 95%
- 3D-Aligner: An advanced computational tool designed to correct image distortion in expansion microscopy for precise 3D reconstitution and quantitative analysis 95%
Similar papers in this journal
- Aberration correction in long GRIN lens-based microendoscopes for extended field-of-view two-photon imaging in deep brain regions 96%
- Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness in awake mice 96%
- Retinal microvascular and neuronal pathologies probed in vivo by adaptive optical two-photon fluorescence microscopy 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.