Molecular markers characterization determining cell fate specification in an adult neurogenesis model of Alzheimer's disease
Blanco, I.; Cabello, J.; Urdanoz-Casado, A.; Acha, B.; Gomez-Orte, E. M.; Roldan, M.; Perez-Rodriguez, D. R.; Mendioroz, M.
Show abstract
Adult hippocampal neurogenesis (AHN) study is still a challenge. In addition to methodological difficulties is the controversy of results derived of human or animal system approaches. In view of the proven link between AHN and learning and memory impairment, we generated a straightforward in vitro model to recapitulate adult neurogenesis in the context of Alzheimer's disease (AD). Neural progenitor cells (NPCs) monolayer culture was differentiated for a period of 29 days and A{beta} peptide 1-42 was administered once a week. mRNA expression of NEUROD1, NCAM1, TUBB3, RBFOX3, CALB1 and GFAP genes was determined by RT-qPCR. Phenotypic changes were observed during directed differentiation. Except for GFAP and CALB1, these changes correlated with altered expression profile of all genes since 9 days. Only TUBB3 expression remained constant while NEUROD1, NCAM1 and RBFOX3 expression increased over time. Moreover, A{beta} treated NPCs showed transient decreases of mRNA expression for NCAM1, TUBB3 and RBFOX3 genes at 9 or 19 days. Our in vitro human NPCs model is framed within the multistep process of AHN in the SGZ of the DG. Remarkably, its transcriptional assessment might reflect alterations detected in AD human patients, deepening our understanding of the disorder and possibly of its pathogenesis.
Matching journals
The top 9 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Live cell imaging of single neurotrophin receptor molecules on human neuron in Alzheimer's disease 96%
- MiR-138-5p upregulation during neuronal maturation parallels with an increase in neuronal survival 95%
- Arginase Inhibition Supports Survival and Differentiation of Neuronal Precursors in Adult Alzheimer's Disease Mice 95%
Similar papers in this journal
- Microglia-secreted TNF-α affects differentiation efficiency and viability of pluripotent stem cell-derived human dopaminergic precursors 96%
- Nicotinamide restricts neural precursor proliferation to enhance catecholaminergic neuronal subtype differentiation from mouse embryonic stem cells 96%
- Cholinergic-like neurons carrying PSEN1 E280A mutation from familial Alzheimers disease reveal intraneuronal Abeta42 accumulation, hyperphosphorylation of TAU, oxidative stress, apoptosis and Ca2+ flux dysregulation: Therapeutic implications 95%
Similar papers in this journal
- Stem cell factor and granulocyte colony-stimulating factor promote remyelination in the chronic phase of severe traumatic brain injury 93%
- Nogo-A regulates the fate of human dental pulp stem cells towards osteogenic, adipogenic, and neurogenic differentiation 93%
- UBA52 is crucial in HSP90 ubiquitylation and neurodegenerative signaling during early phase of Parkinson disease 93%
Similar papers in this journal
- Hotair and Malat1 Long Noncoding RNAs regulate Bdnf Expression and Oligodendrocyte Precursor Cells Differentiation 95%
- Cellular and Extracellular microRNA Dysregulation in LRRK2-Linked Parkinson's Disease 94%
- Apoptotic factors and mitochondrial complexes assist determination of strain-specific susceptibility of mice to Parkinsonian neurotoxin MPTP 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.