Membrane voltage-dependent activation of the flagellar protein export engine
Minamino, T.; Morimoto, Y. V.; Kinoshita, M.; Namba, K.
Show abstract
Ion motive force (IMF) consists of the electric potential difference ({Delta}{Psi}) and the ion concentration difference ({Delta}pI) across the cytoplasmic membrane. The flagellar protein export machinery is an ion/protein antiporter utilizing IMF to drive ion-coupled protein export, but it remains unknown how. Here, we report a {Delta}{Psi}-dependent activation mechanism of the transmembrane export gate complex. Depletions of both H+ and Na+ gradients nearly diminished flagellar protein export in the absence of the cytoplasmic ATPase complex, but an increase in {Delta}{Psi} by an upward shift of external pH from 7.5 to 8.5 dramatically recovered it. An increase in the cytoplasmic level of export substrates and gain-of-function mutations in FlhA enhanced protein export at external pH 7.5 in the absence of Na+ in a similar manner to {Delta}{Psi} increase. We propose that the export gate complex has a voltage-gated mechanism to activate the ion/protein antiporter of the flagellar protein export engine.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Proteins with proximal-distal asymmetries in axoneme localisation control flagellum beat frequency 95%
- Primary role of the Tol-Pal complex in bacterial outer membrane lipid homeostasis 95%
- Glycosylation of serine/threonine-rich intrinsically disordered regions of membrane-associated proteins in streptococci 95%
Similar papers in this journal
- Periplasm homeostatic regulation maintains spatial constraints essential for cell envelope processes and cell viability 95%
- Recognition of discrete export signals in flagellar subunits during bacterial Type III secretion 94%
- A conserved cell-pole determinant organizes proper polar flagellum formation 94%
Similar papers in this journal
- Three PilZ domain proteins, PlpA, PixA and PixB, have distinct functions in regulation of motility and development in Myxococcus xanthus 94%
- Regulation of bacterial cell cycle progression by redundant phosphatases 93%
- The division defect of a Bacillus subtilis minD noc double mutant can be suppressed by Spx-dependent and Spx-independent mechanisms 93%
Similar papers in this journal
Similar papers in this journal
- Tyrosine phosphorylation-dependent localization of TmaR, a novel E. coli polar protein that controls activity of the major sugar regulator by polar sequestration 94%
- DprA recruits ComM to facilitate recombination during natural transformation in Gram-negative bacteria 94%
- Oxygen depletion and nitric oxide stimulate type IV MSHA pilus retraction in Vibrio cholerae via activation of the phosphodiesterase CdpA 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.