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Single species RNA purification with DNA nanoswitches

Zhou, L.; Cavaliere, C.; Hayden, A.; Dey, P.; Mao, S.; Chandrasekaran, A. R.; Sheng, J.; Dey, B. K.; Halvorsen, K.

2020-07-07 molecular biology
10.1101/2020.07.07.191338 bioRxiv
Show abstract

We report a novel method to purify individual RNA species using programmable DNA nanoswitches to facilitate RNA capture, release, and isolation. We validate sequence-based purification of microRNA, rRNA, and an mRNA fragment from total RNA, demonstrate multiplexing with two different RNA species, and show an application with downstream LC/MS analysis to identify RNA modifications. The simplicity, low cost, and low sample requirements of our method make it suitable for easy adoption.Competing Interest StatementLZ, ARC, and KH have intellectual property related to DNA nanoswitches. All other authors declare no competing interests.View Full Text

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