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Most of Tobacco Male Meiocytes Are Involved in Intercellular Nuclear Migration at the First Meiotic Prophase

Mursalimov, S.; Ohno, N.; Matsumoto, M.; Bayborodin, S.; Deineko, E.

2020-07-07 cell biology
10.1101/2020.07.07.188854 bioRxiv
Show abstract

Serial block-face scanning electron microscopy was used here to study tobacco male meiosis. Three-dimensional ultrastructural analyses revealed that intercellular nuclear migration (INM) occurs in 90-100% of tobacco meiocytes. At the very beginning of meiosis, every meiocyte connected with neighboring cells by more than 100 channels was capable of INM. At leptotene and zygotene, the nucleus in most tobacco meiocytes approached the cell wall and formed nuclear protuberances (NPs) that crossed the cell wall through the channels and got into the cytoplasm of a neighboring cell. The NPs did not separate from the migrating nuclei and never produced micronuclei. Approximately 70% of NPs reached nuclei of neighboring cells. The NPs and the nuclei they reached got very close, and the gap between their nuclear membranes became indistinguishable in some cases. At pachytene, NPs detached from the nuclei of neighboring cells and came back into their own cells. After that, the INM stopped. The reason for such behavior of nuclei is unclear. INM probably causes a short-lived fusion of two nuclei and thus has a potential to form aneuploid or unreduced pollen. We consider INM a normal part of tobacco meiosis.Competing Interest StatementThe authors have declared no competing interest.View Full Text

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