USP11 deubiquitinates monoubiquitinated SPRTN to repair DNA-protein crosslinks
Perry, M.; Kollala, S. S.; Biegert, M.; Su, G.; Kodavati, M.; Mallard, H.; Kreiling, N.; Holbrook, A.; Ghosal, G.
Show abstract
SUMMARYDNA-protein crosslinks (DPCs) are toxic DNA lesions that interfere with DNA metabolic processes such as replication, transcription and recombination. SPRTN is a replication-coupled DNA-dependent metalloprotease that cleaves proteins crosslinked to DNA to promote DPC repair. SPRTN function is tightly regulated by a monoubiquitin switch that controls SPRTN chromatin accessibility during DPC repair. The deubiquitinase regulating SPRTN function in DPC repair is unknown. Here, we identify USP11 as a SPRTN deubiquitinase. USP11 interacts with SPRTN and cleaves monoubiquitinated SPRTN in cells and in vitro. USP11 depletion impairs SPRTN deubiquitination in response to formaldehyde-induced DPCs. Loss of USP11 causes an accumulation of unrepaired DPCs and cellular hypersensitivity to treatment with DPC-inducing agents. Our findings elucidate the function of USP11 in the regulation of SPRTN monoubiquitination and SPRTN-mediated DPC repair.Competing Interest StatementThe authors have declared no competing interest.View Full Text
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- REV7 associates with ATRIP and inhibits ATR kinase activity 96%
- Rrp1 translocase and ubiquitin ligase activities restrict the genome destabilising effects of Rad51 in fission yeast 95%
- CRISPR knockout genome-wide screens identify the HELQ-RAD52 axis in regulating the repair of cisplatin-induced single stranded DNA gaps 95%
Similar papers in this journal
- Differential transcriptional activity of ΔNp63β is encoded by an isoform-specific C-terminus 94%
- Histone chaperone Nucleophosmin regulates transcription of key genes involved in oral tumorigenesis. 93%
- The RecQ DNA helicase Rqh1 promotes Rad3ATR kinase signaling in the DNA replication checkpoint pathway of fission yeast 93%
Similar papers in this journal
- Comprehensive mapping of cell fates in microsatellite unstable cancer cells support dual targeting of WRN and ATR 94%
- The ribosome-associated quality control factor TCF25 imposes K48 specificity on Listerin-mediated ubiquitination of nascent chains by binding and specifically orienting the acceptor ubiquitin 93%
- SUMO2 Promotes Histone Pre-mRNA Processing by Stabilizing Histone Locus Body Interactions and Facilitating U7 snRNP Assembly 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.