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Quantum dot probes for the quantitative study of drug transport by the MacAB TolC efflux pump in lipid scaffolds

Souabni, H.; Batista dos Santos, W.; Cece, Q.; Puvanendran, D.; Picard, M.

2020-06-17 biochemistry
10.1101/2020.06.16.154831 bioRxiv
Show abstract

ABC tripartite efflux pumps are macromolecular membrane protein machineries that expel a large variety of drugs and export virulence factors from Gram negative bacteria. Using a lipid scaffold mimicking the two-membrane environment of the transporter and designing spectroscopic conditions allowing the monitoring of both ATP hydrolysis and substrate transport in real time, we show that MacAB-TolC accommodates transport and energy consumption with high coupling efficiency.

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