Bridging scales in scattering tissues via multifocal two-photon microscopy
Chen, D.; Segovia-Miranda, F.; Walker, N.; Valenzuela, J. I.; Zerial, M.; Myers, E. W.
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Imaging biological systems at subcellular resolution and across scales is essential to under-standing how cells form tissues, organs, and organisms. However, existing large-scale optical techniques often require harsh tissue-clearing methods that cause significant morphological changes, compromise the integrity of cell membranes, and reduce the signal of fluorescent proteins. Here, we demonstrate multifocal two-photon microscopy that enables imaging mesoscopic scattering samples in their native tissue environment at high resolution and high speed.
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