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A New Bioinformatic Tool To Interpret Metagenomics /Metatranscriptomics Results Based On The Geometry Of The Clustering Network And Its Differentially Gene Ontologies (Gango)

MONLEON GETINO, A.; Paytuvi-Gallart, A.; Sanseverino, W.; Mendez, J.

2020-06-10 bioinformatics
10.1101/2020.06.10.140103 bioRxiv
Show abstract

High-throughput experimental techniques, such as metagenomics or metatranscriptomics, produce large amounts of data, which interpretation and conversion into understandable knowledge can be challenging and out of reach. We present GANGO, a new algorithm based on the ecological concept of consortium (groups biologically connected) and by using clustering network analysis, gene ontologies and powerful hypothesis test allows the identification and interpretation of complex ecological networks, allowing the identification of the relationship between taxa/genes, the number of groups, their relations and their functionalities using the annotated genes of an organism in a database (e.g. UniProt or Ensembl). Three examples of the use of GANGO are shown: a simulated mixture of fungi and bacteria, alterations in soil fungi communities after a diesel-oil spill and genomic changes in Saccharomyces cerevisae due to abiotic stress.

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