Human iPSC-derived alveolar and airway epithelial cells can be cultured at air-liquid interface and express SARS-CoV-2 host factors
Abo, K. M.; Ma, L.; Matte, T.; Huang, J.; Alysandratos, K. D.; Werder, R. B.; Mithal, A.; Beermann, M. L.; Lindstrom-Vautrin, J.; Mostoslavsky, G.; Ikonomou, L.; Kotton, D. N.; Hawkins, F.; Wilson, A.; Villacorta-Martin, C.
Show abstract
Development of an anti-SARS-CoV-2 therapeutic is hindered by the lack of physiologically relevant model systems that can recapitulate host-viral interactions in human cell types, specifically the epithelium of the lung. Here, we compare induced pluripotent stem cell (iPSC)-derived alveolar and airway epithelial cells to primary lung epithelial cell controls, focusing on expression levels of genes relevant for COVID-19 disease modeling. iPSC-derived alveolar epithelial type II-like cells (iAT2s) and iPSC-derived airway epithelial lineages express key transcripts associated with lung identity in the majority of cells produced in culture. They express ACE2 and TMPRSS2, transcripts encoding essential host factors required for SARS-CoV-2 infection, in a minor subset of each cell sub-lineage, similar to frequencies observed in primary cells. In order to prepare human culture systems that are amenable to modeling viral infection of both the proximal and distal lung epithelium, we adapt iPSC-derived alveolar and airway epithelial cells to two-dimensional air-liquid interface cultures. These engineered human lung cell systems represent sharable, physiologically relevant platforms for SARS-CoV-2 infection modeling and may therefore expedite the development of an effective pharmacologic intervention for COVID-19.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Cryobanking of human distal lung epithelial cells for preservation of their phenotypic and functional characteristics. 94%
- Novel porcine model reveals two distinct LGR5 cell types during lung development and homeostasis 93%
- Intermediary role of lung alveolar type 1 cells in epithelial repair upon Sendai virus infection 93%
Similar papers in this journal
- Decoding the IGF1 Signaling Gene Regulatory Network Behind Alveologenesis from A Mouse Model of Bronchopulmonary Dysplasia 94%
- GSK3 inhibition rescues growth and telomere dysfunction in dyskeratosis congenita iPSC-derived type II alveolar epithelial cells 94%
- Atf3 defines a population of pulmonary endothelial cells essential for lung regeneration 93%
Similar papers in this journal
- Simultaneous isolation of proximal and distal lung progenitor cells from individual mice using a 3D printed guide reduces proximal cell contamination of distal lung epithelial cell isolations 96%
- Conjunctival epithelial cells resist productive SARS-CoV-2 infection 93%
- Rostrocaudal Patterning and Neural Crest Differentiation of Human Pre-Neural Spinal Cord Progenitors in vitro 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.