Robust estimates of cuticle conductance on stomatous leaf surfaces during the light induction of photosynthesis

O_LICuticle conductance (gcw) can bias calculations of intercellular CO2 concentration inside the leaf (Ci) when stomatal conductance (gsw) is small. C_LIO_LIWe examined how the light induction of photosynthesis impacts calculations by directly measuring Ci along with standard gas exchange in sunflower and tobacco leaves. C_LIO_LIWhen photosynthesis was induced from dark to saturating light (1200 mol m-2 s-1 PAR) the calculated Ci was significantly larger than measured Ci and the difference decreased as gsw increased. This difference could lead to over-estimation of rubisco deactivation by limited CO2 supply during early induction of photosynthesis. However, only small differences in Ci were observed during the induction from shade (50 mol m-2 s-1 PAR) because gsw was sufficiently large. The induction from dark also allowed robust estimations of gcw when combined with direct Ci measurements. These gcw estimates succeeded in correcting the calculation, suggesting that the cuticle was the major source of error. C_LIO_LIDespite a technical restriction to amphi-stomatous leaves, the presented technique has a potential to provide insights into the cuticle conductance on intact stomatous leaf surfaces. C_LI